DNA
Prefix

Part:BBa_G00000:Design

Designed by: Reshma Shetty   Group: BE.109 Instructors   (2006-03-13)

BioBrick cloning site prefix


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NotI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Please see Tom Knight's technical report for design notes on this part.

Source

References

  • T. F. Knight. [http://hdl.handle.net/1721.1/21168 Idempotent vector design for standard assembly of BioBricks]. Technical report, MIT DSpace archive, 2003.